Benzo[]coumarin-Based Fluorescent Probes for Bioimaging Applications

Figure 12

In vivo TPM coimaging of MAO activity and Aβ plaques using probe 32. (a) In vivo fluorescence images (from (z)-stack, magnified 20x) of the frontal cortex region of transgenic and healthy mice, obtained after intraperitoneal injection of probe 32. The scale bar is 60 μm. The images were acquired at 200–300 μm depth from the surface of the cortex. (b) Plots of the average fluorescence intensity of Aβ plaques and background images in (a), respectively. (c) A plot of the background fluorescence intensity versus the plaque volume (μm³) per mm³. p-value . Reproduced from [43] with permission from the American Chemical Society, copyright 2016.

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