A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

Figure 6

Confocal fluorescence images of living HeLa cells. Bright-field image (a) and fluorescence image (b) of cells incubated with probe 1 (10 μM) for 1 h; (c) overlay of the images of (a) and (b); bright-field image (d) and fluorescence image (e) of cells incubated with probe 1 (10 μM) for 1 h and subsequent treatment with Cys (100 μM) for another 1 h; (f) overlay of the images of (d) and (e). λ_ex = 405 nm; scare bar = 25 μm.

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