Research Article
LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
Table 1
Composition of the saponified sea buckthorn seed oil determined by HPLC-NMR.
| Compound | Comparative contenta | Overall content in the seed oilb | Retention time (min)c |
| -avenasterol | 13% |
0.3% | 24 | Unknown I | 2% | <0.1% | 25 | Unknown II (-sterol) | 1% | <0.1% | 25 |
Cykloeukalenol | 4% | 0.1% | 26 | -amyrin | 4% | 0.1% | 27 | Campesterol | 2% | <0.1% | 27 | -amyrin | 3% | <0.1% | 28 | -sitosterol | 69% | 1.7% | 29 | Sitostanol | 2% | <0.1% | 32 |
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Molar ratio in the phytosterol fraction, bweight ratio in the extract sample, and cisocratic method (CDCl3 : acetonitrile, 25 : 75).
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