LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Table 1

Composition of the saponified sea buckthorn seed oil determined by HPLC-NMR.

Compound	Comparative; content^a	Overall content in; the seed oil^b	Retention; time (min)^c
Δ^5-avenasterol	13%	0.3%	24
Unknown I	2%	<0.1%	25
Unknown II; (Δ^7-sterol)	1%	<0.1%	25
Cykloeukalenol	4%	0.1%	26
𝛼-amyrin	4%	0.1%	27
Campesterol	2%	<0.1%	27
𝛽-amyrin	3%	<0.1%	28
𝛽-sitosterol	69%	1.7%	29
Sitostanol	2%	<0.1%	32

aMolar ratio in the phytosterol fraction, ^bweight ratio in the extract sample, and ^cisocratic method (CDCl₃ : acetonitrile, 25 : 75).