Increased Expression of RhoA in Epithelium and Smooth Muscle of Obese Mouse Models: Implications for Isoprenoid Control of Airway Smooth Muscle and Fibroblasts
Figure 3
Mevastatin inhibits ASM and NHLF proliferation. Airway smooth muscle cell (ASM, shown as solid lines) and normal human lung fibroblast (NHLF shown as dotted lines) proliferation are decreased in a dose-dependent fashion by mevastatin (■). This effect is reduced somewhat by FPP (●) and is almost abolished by GGPP (▲), suggesting specificity to the Rho family of GTPases. ASM cell proliferation assayed by (a) MTS tetrazolium compound reduction (CellTiter, Promega) and (b) BrdU incorporation. HLF fibroblast proliferation assayed by (c) MTS tetrazolium compound reduction and (d) BrdU incorporation. Data shown are the mean (±SE) of three experiments, each performed in triplicate. Groups are compared by ANOVA with the Bonferroni test; compared to untreated, compared to mevastatin treatment alone.
