Visualization of p53 localization in CdCl2-treated HepG2 cells. Fixed and p53 stained cells were visualized by indirect probing with Alexa Fluor antibody. In control HepG2 (a), a spread distribution of the p53 is shown throughout the cells, while the treatment with Cd increases the nuclear localization of the protein (d, g). (a, d, g) cells probed for p53 in controls (a), 2 μM (d), and 10 μM (g) Cd concentrations. (b, e, h) cells stained with DAPI for the nuclei visualization in controls (b), 2 μM (e), and 10 μM (h) Cd concentrations. Merge is represented in (c, f, i) images. Microscopy magnification: 400x.
