Aptamer-Based Strategies to Address Challenges in COVID-19 Diagnosis and Treatments

Table 2

List of high-affinity ssDNA and RNA aptamers targeting N protein or RBD of the S protein isolated using different SELEX protocols as diagnostic agents for COVID-19.

Target	Types	Aptamer sequence (5′-3′)	Randomization region length (bp)	KD (nM)	Partitioning method	Selection pool regeneration method	References
N Protein	ssDNA	GCA​ATG​GTA​CGG​TAC​TTC​CGG​ATG​CGG​AAA​CTG​GCT​AAT​TGG​TGA​GGC​TGG​GGC​GGT​CGT​GCA​GCA​AAA​GTG​CAC​GCT​ACT​TTG​CTA​A	45	—	—	—	Chen et al. [2]
N Protein	ssDNA	GCT​GGA​TGT​CGC​TTA​CGA​CAA​TAT​TCC​TTA​GGG​GCA​CCG​CTA​CAT​TGA​CAC​ATC​CAG​C	36	0.49 ± 0.05	—	—	Zhang et al. [5]
		GCT​GGA​TGT​CAC​CGG​ATT​GTC​GGA​CAT​CGG​ATT​GTC​TGA​GTC​ATA​TGA​CAC​ATC​CAG​C		0.70 ± 0.06
		GCT​GGA​TGT​TGA​CCT​TTA​CAG​ATC​GGA​TTC​TGT​GGG​GCG​TTA​AAC​TGA​CAC​ATC​CAG​C		2.74 ± 0.08
		GCT​GGA​TGT​TCA​TGC​TGG​CAA​AAT​TCC​TTA​GGG​GCA​CCG​TTA​CTT​TGA​CAC​ATC​CAG​C		4.38 ± 0.06
SRBD	ssDNA	CAG​CAC​CGA​CCT​TGT​GCT​TTG​GGA​GTG​CTGGTC​CAA​GGG​CGT​TAA​TGG​ACA	40	5.8	Bead	Magnetic separation using streptavidin-coated bead	Song et al. [4]
		ATC​CAG​AGT​GAC​GCA​GCA​TTT​CAT​CGG​GTCCAA​AAG​GGG​CTG​CTC​GGG​ATT​GCG​GAT​ATG​GAC​ACG​T		19.9