Nanogels Containing <i>Foeniculum vulgare</i> Mill. and <i>Mentha piperita</i> L. Essential Oils: Mosquitoes’ Repellent Activity and Antibacterial Effect

Sanei-Dehkordi, Alireza; Abdollahi, Abbas; Safari, Mojdeh; Karami, Farnaz; Ghaznavi, Ghazal; Osanloo, Mahmoud

doi:https://doi.org/10.1155/2022/4510182

Interdisciplinary Perspectives on Infectious Diseases

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Abstract Introduction Materials and Methods Results Discussion Conclusions Data Availability Ethical Approval Consent Conflicts of Interest Authors’ Contributions Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 4510182 | https://doi.org/10.1155/2022/4510182

Nanogels Containing Foeniculum vulgare Mill. and Mentha piperita L. Essential Oils: Mosquitoes’ Repellent Activity and Antibacterial Effect

Alireza Sanei-Dehkordi,^1,2Abbas Abdollahi,³Mojdeh Safari,⁴Farnaz Karami,⁵Ghazal Ghaznavi,⁵and Mahmoud Osanloo^6,7

Academic Editor: Payam Behzadi

Received17 Jul 2022

Accepted08 Aug 2022

Published31 Aug 2022

Abstract

Foeniculum vulgare Mill. and Mentha piperita L. are two common medicinally important plants with a wide range of biological activities such as insecticide and antibacterial effects. In this study, the chemical composition of their essential oils was investigated using GC-MS analysis. After that, their nanoemulsions were prepared; optimum samples with droplet sizes of 74 ± 7 and 136 ± 5 nm were gelified. The viscosity of the prepared nanogels and the successful loading of the essential oil in them were investigated. The efficacy of the nanogel containing M. piperita essential oil as a repellent and antibacterial agent was more potent than the nanogel containing F. vulgare essential oil. Its completely protected time against Anopheles stephensi, the main malaria mosquito vector, was 120 ± 8 min. Moreover, the growth of Escherichia coli and Staphylococcus aureus after treatment with 5000 µg/mL of nanogel containing M. piperita essential oil was reduced by 100 and 65%, respectively. Considering natural constituents, a straightforward preparation method, and high efficacy, the nanogel containing M. piperita essential oil could be introduced for further investigation against other mosquitoes and bacterial species.

1. Introduction

Essential oils are volatile natural oils formed as secondary metabolites in aromatic plants [1]. They are extracted from different parts of plant organs, e.g., buds, flowers, leaves, stems, twigs, seeds, fruits, roots, wood, or bark [2]. They have many biological effects, such as antiseptic, antibacterial, antiviral, and fungicidal properties [3, 4]. Besides, their larvicidal activity, repellent effects, and insecticide properties have also been confirmed [5, 6]. However, they should be stabilized due to their volatility and instability [7]. Preparation of EO-based nanoformulation has been recently considered a promising approach [8]. Among the common nanoformulations such as nanoemulsions, polymeric nanoparticles, and lipid nanocarriers, nanogels have received much attention, especially in topical applications, due to proper viscosity, high loading capacity, biocompatibility, and biodegradability [9, 10].

Escherichia coli and Staphylococcus aureus are two Gram-negative and positive opportunistic pathogens that (could) cause severe and life-threatening human infections [11]. S. aureus is mainly responsible for postoperative wound infection, toxic shock syndrome, and food poisoning [12]. The E. coli is present in the human intestine and causes lower urinary tract infection, coleocystis, or septicemia [13]. These bacteria enter the body through contaminated hands and can, of course, cause skin damage. Moreover, S. aureus is an important cause of soft tissue and skin infections such as boils, impetigo, carbuncles, staphylococcal scalded skin syndrome, and cellulitis.

Furthermore, malaria, with about 241 million cases and 627,000 deaths in 2020, is still the most dreadful of mosquito-borne diseases [14]. Around 30 species of the 400 identified Anopheles mosquito species are the vectors of malaria to humans [15]. Anopheles stephensi Liston is one of the most important malaria vectors in the Middle East and South Asia [16, 17]. However, it has recently expanded to Ethiopia, Djibouti, Lakshadweep, and Sri Lanka [18]. Besides, mosquito bites can be infected by other pathogens such as bacteria. However, one recommended solution to prevent mosquito-borne disease transmission is to use repellents [19, 20]. On the other hand, resistance to industrial repellents and their adverse effects has been one of the challenges for health systems in recent years [21, 22]. For instance, DEET (N, N-diethyl-3 methylbenzamide) is one of the best known and most successful synthetic chemical repellents that, by blocking the olfactory receptors neurons, causes repellency [23, 24]. However, its usage has been questioned due to its side effects on humans such as hypotension, seizures, neurotoxic, and skin irritations [25, 26].

Foeniculum vulgare Mill. and Mentha piperita L. are two common medicinally important plants with a wide range of biological activities such as antibacterial and insecticide effects [27, 28]. For instance, the literature reported that F. vulgare EO at a concentration of 40 mg/L caused 50% mortality for the second instars larvae Culex pipiens [29]. Besides, a protection time of 0.5% M. piperita EO against An. stephensi was reported at 17 min [30].

This study was an attempt to prepare multifunctional topically administrated natural nanogels. First, two nanogels containing F. vulgare and M. piperita EOs were prepared. After that, their antibacterial activities against E. coli and S. aureus were investigated. Finally, their repellent efficacies were investigated against An. stephesni compared to DEET as a gold standard repellent.

2. Materials and Methods

2.1. Materials

F. vulgare and M. piperita EOs were bought from Tabib Daru Company (Iran) and Zardband Pharmaceuticals Company (Iran). S. aureus (ATCC 25923) and E. coli (ATCC 25922) were provided by the Pasteur Institute of Iran. Carboxymethylcellulose (CMC), Mueller–Hinton broth, Mueller–Hinton agar, and Tween 20 were bought from Merck Chemicals (Germany). DEET 40% was purchased from Reyhan Naghsh Jahan Pharmaceutical Co., Iran. It was diluted to 2.0% using distilled water as the diluent following the formulated nanogel products.

2.2. Chemical Composition of the EOs

A gas chromatography device (Agilent 6890, HP-5MS column, USA) connected to a mass spectrometer (Agilent 5973, USA) was used for chemical compositions of the EOs as described in our previous study. Besides, relative abundances were calculated by peak area normalization [31].

2.3. Preparation and Characterization of Nanoemulsion-Based Gels

A fixed amount of (2.0% v/v) each EO was mixed with different amounts of Tween 20 (2000 rpm, 3 min, room temperature) to form a homogenous mixture. Distilled water was then added dropwise up to the final volume (5000 µL) and stirred for 40 minutes. The prepared nanoemulsions were subjected to size analysis using a DLS-type apparatus (K-One Nano Ltd., Korea). Nanoemulsions with proper size characteristics, including droplet size of <200 nm and droplet size distribution (SPAN) less than 1 [32], were considered optimum samples.

An optimum nanoemulsion from each EO (No. 1 and No. 7,Table 1) was selected for gelation; CMC (3.5% w/v) was added to each and stirred (2000 rpm) overnight at room temperature to complete the gelation process. The prepared nanogels containing F. vulgare and M. piperita EO were abbreviated as FVNG and MPNG. A schematic of the described method is depicted in Figure 1. Furthermore, blank gels of each nanogel were also prepared using the same approach, only without EO.

The viscosity of FVNG and MPNG at shear rates of 0.1–100 1/s was investigated using a Rheometer machine (MCR-302, Anton Paar, Austria). Moreover, ATR-FTIR analysis was used to investigate the successful loading of the EOs in the nanogels; spectra of EOs, blank gels, and nanogels (FVNG and MPNG) were recorded in a wavenumber range of 400–3900 cm⁻¹. Without any preparation process, the samples were subjected to the spectrometer apparatus (Tensor II model, Bruker Co, Germany).

2.4. Repellent Bioassay

Susceptible mosquitoes (Bandar-e-Abbas strain) were used in the current study. They were reared and maintained at 27 ± 2 C temperature, ≥70 ± 10% relative humidity, and a 12 : 12 (light: dark) photoperiod. For repellent bioassays, 250 nonblood fed and nulliparous 5–7 days old adult female mosquitoes were kept in cages (40 × 40 × 40 cm) and not fed for 14 h before repellency tests. A 47-year-old male volunteer was employed to determine the protection time using the Arm-in-cage method with a slight modification [33]. His forearm was first washed with 70% alcohol and dried with a towel. Only the underside of the lower arm between the wrist and elbow, with an area of 8 cm × 12.5 cm (covered by fewer hairs), was exposed, and latex gloves covered the hand. The volunteer’s hands were then impregnated with the samples (1g of FVNG and MPNG, 1 mL of DEET). After 5 minutes, the volunteer placed his forearm in a cage for 3 minutes. This procedure is repeated at a 30-minute interval; the test was stopped when one landing and/or probing occurred in a 3 min test.

2.5. Antibacterial Tests

For investigation of antibacterial tests of FVNG and MPNG as well as their blank gels, the ATCC100 assay was used with slight modifications [32]. Four mL of each fresh bacterial suspension (2 × 10⁵ CFU/mL) was first filled separately in 6 cm plate dishes. Then, by adding 1.0, 0.5, and 0.25 g of each nanogel, final concentrations of EOs were fixed at 5000, 2500, and 1250 µg/mL. Antibacterial effects of blank gels were also investigated in a similar process. The treated plates were then incubated at 37°C for 24 h, and 10 µL of suspensions was cultured on a Muller–Hinton agar culture plate and incubated for 24 h. Finally, the number of grown colonies was counted and compared with the control group, and growth reduction was calculated using the following equation.

2.6. Statistical Analyses

All experiments were repeated three times, and the results were given as mean ± standard deviation. For the comparison of two or higher samples, the independent sample t-test and one-way ANOVA with at least a 0.05 significance level were used (STATA v11, StataCorp, USA).

3. Results

3.1. Compounds of F. vulgare and M. piperita EOs

Identified compounds in the EOs using GC-MS analysis are listed in Table 2. trans-Anethole (52.6%), limonene (11.1%), carvone (8.2%), tarragon (7.7%), and fenchone (4.5%) are five major compounds in F. vulgare EO. Besides, menthol (31.1%), menthone (22.1%), camphane (7.0%), menthofuran (6.0%), and iso-menthone (5.9%) are major compounds in M. piperita EO.

3.2. Ten Prepared Nanoemulsions and Two Nanoemulsion-Based Nanogels

Five nanoemulsions containing 2.0% F. vulgare EO were prepared (Table 2, Nos. 1–5). Sample No. 1, with proper size characteristics, i.e., droplet size of 74 ± 7 nm and SPAN of 0.97, was selected as the optimum sample for gelation. In addition, five nanoemulsions containing M. piperita EO (Table 2, Nos. 6–10) were prepared; No. 7 with 136 ± 5 nm droplet size and 0.96 SPAN was selected as the optimum nanoemulsion for gelation. DLS profiles of two selected nanoemulsions are depicted in Figure 2.

Two of the mentioned nanoemulsions were gelified using 3.5% w/v CMC. Their viscosity at different shear rates (0.1–100 1/s) was fully fitted with a well-known non-Newtonian regression, the Carreau–Yasuda model (Figure 3). In non-Newtonian fluids, viscosity decreases with an increasing shear rate [34].

(a)

(b)

3.3. Successful Loading of the EOs in Nanogels

In the ATR-FTIR spectrum of F. vulgare EO (Figure 4(a)), the absorption peaks at around 2834–3022 cm⁻¹ are associated with stretching –OH and NH₂ groups. The peaks observed at 1737 cm⁻¹ and 1607 cm⁻¹ are assigned to the stretching vibration of the –C=O group. Several characteristic peaks appeared at 1509 cm⁻¹ (-NH is plane bend and –CN stretching), 1414, 1440, and 1243 cm⁻¹ (-NH bending and –CN stretching), 1035–1174 cm⁻¹ (aromatic C–H in the plane bend), and 837 cm⁻¹ (out of the plane –NH bending). A similar observation for F. vulgare EO was reported in the literature [35]. In the spectra, blank gel (Figure 4(b)), the prominent peak at 3508–3698 cm⁻¹ is assigned to the OH group in CMC [36]. The bands that appeared at 2922, 1581, and 1461 cm⁻¹ are related to the stretching vibration of C-H, C=O stretching, and hydrocarbon groups (-CH₂), respectively, in CMC. The peak at 1080–1252 cm⁻¹ is attributed to the CMC’s ether groups (-O- stretching). In F. vulgare, nanogel (FVNG) (Figure 4(c)) retained most of the peaks that appeared in the spectra of blank gel and F. vulgare EO confirmed the loading of the EO into the nanogel structure, although some changes in the position and intensity of peaks were identified.

In the spectrum of M. piperita (Figure 4(d)), different functional groups such as alkanes, phenols, alkenes, ethers, alcohol, ester, and carboxylic acid are observed [37, 38]. The major peak at 3400 cm⁻¹ is attributed to the hydrogen-bonded alcohol and phenols. The bands observed at 2869, 2922, 2953, 1287, 1368, and 1455 cm⁻¹ are assigned to alkanes’ C–H stretching. The absorption peak observed at 1710 cm⁻¹ is related to C=C starching. The bands that appeared at 1044 and 1079 cm⁻¹ are attributed to the C–O vibration of ethers, alcohol, esters, and carboxylic acids. The characteristic band around 875 cm⁻¹ could be related to alkenes’ C–H bonds. The main absorption peaks of blank gel (Figure 4(e)) of this nanogel are like previous blank gel and interpreted above. In the FTIR spectrum of M. piperita EO nanogel (Figure 4(f)), some peaks’ changes in shape and intensity were identified, which correspond to the possible interaction between M. piperita EO and CMC. For instance, the absorption band at 1581 cm⁻¹ attributed to C=O in CMC was shifted to 1579 cm⁻¹ in the spectrum of prepared nanogel. Moreover, the intensity of the peaks observed at 2869, 2922, and 2953 cm⁻¹ assigned to the C–H stretching of alkanes in M. piperita EO was changed in the spectrum of the final nanogel. Finally, the presence of characteristic peaks of both CMC and M. piperita EO in the FTIR spectra of MPNG indicates both components’ existence in the structure of obtained nanogels.

3.4. Repellent Properties of the Nanogels

From Figure 5, MPNG, with a complete protection time of 120 ± 8 min, was significantly more potent than FVNG (70 ± 6). However, its efficacy was less than DEET, with a protection time of 140 ± 8 (). Besides, both blank gels with 3 min of complete protection time did not show proper efficacy.

3.5. Antibacterial Effects of the Nanogels

The antibacterial effects of the nanogels and their blank gels against E. coli and S. aureus are depicted in Figures 6 and 7. The growth of E. coli after treatment with 1250, 2500, and 5000 µg/mL of FVNG was not significantly reduced (growth 92%). However, the growth of S. aureus after treatment with 5000 µg/mL FVNG was reduced by 30%.

Besides, after treatment with MPNG 1250, 2500, and 5000 µg/mL, the growth of E. coli was substantially reduced ( 96%). However, after treating S. aureus at those concentrations, bacterial growth was observed at 70, 61, and 35%. Moreover, both nanogels did not affect the growth of both bacterial types.

4. Discussion

Mosquitoes (Diptera: Culicidae) transmit malaria, dengue, yellow fever, encephalitis, filariasis, chikungunya, and Zika virus [39, 40]. Indoor residual spraying and insecticide-impregnated bed nets are core components of malaria prevention and elimination strategies, and repellents are also recommended in endemic regions [41, 42]. Repellents are substances that deter mosquitoes (or insects) from flying to, landing on, or biting human, animal skin, and surfaces [43, 44]. Due to mosquitoes’ resistance to industrial repellents and their adverse effects on human health, many attempts have recently been made to develop natural nanorepellents. For instance, a solid lipid nanoparticle containing 1% Zataria multiflora EO was introduced with a 90 min protection time against An. stephensi [45]. Besides, the literature reported nanoemulsions containing 15% Eucalyptus globulus EO with 170 min protection time against a mixture of mosquitoes [46]. Moreover, nanoemulsion containing 50% M. piperita EO with 257 min protection time against An. stephensi was also reported [47].

In developing essential oil-based repellents, controlling the pungent odor of EOs is a challenge; the corresponding author of this article has observed that volunteers refuse to use pungent odor repellents. A practical solution to control the odor of colloidal nanoformulations (such as nanoemulsion or nanoparticles) is to turn them into nanogels. Besides, their topical application is also facilitated due to the increased viscosity. Therefore, in the current study, nanogels dosage form was used; interestingly, MPNG showed a 120 min repellent effect against An. stephensi.

Symbiotic and opportunistic bacteria commonly influence the skin as the body’s first barrier against environmental pathogens; they can cause pain, swelling, and skin color changes [48, 49]. Moreover, some opportunistic bacteria could enter the body through open wounds, possibly leading to bloodstream infections like septicemia [50, 51]. In the current study, the growth of E. coli after treatment with 1250 µg/mL MPNG was reduced by more than 95%. On the other hand, the efficacy of MPNG against S. aureus was less than E. coli; the growth after treatment with 5000 µg/mL was reduced by 65%. Some reports with promising antibacterial effects of nonformulated EOs and nanostructures containing EOs have been found in the literature. For instance, the antibacterial activity of Juniper communis EO against standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was investigated [52]. Besides, the viability of S. aureus and E. coli after 24 h exposure to polycaprolactone nanofibers containing Mentha piperita EO was decreased to around 50% [53]. Moreover, the E. coli colonies were reduced by about 4.0 log CFU/mL after treatment with thyme EO nanoemulsion [54].

5. Conclusions

This study aimed to develop two natural nanogels using F. vulgare and M. piperita EOs as mosquito repellent and antibacterial agent prototypes. The complete protection times of the nanogels against An. stephensi were observed as 70 (±6) and 120 (±8) min. The nanogel containing F. vulgare EO showed some degree of antibacterial effects against E. coli and S. aureus. However, after treatment with nanogel of M. piperita EO 100 and 65%, their growth was reduced. Therefore, the nanogel of M. piperita EO could be considered for further investigations as mosquito repellent and antibacterial agent.

Data Availability

The data used to support this study are available from the corresponding author upon request.

Ethical Approval

This study was ethically approved by the Ethical Committee of Fasa University of Medical Sciences, IR.FUMS.REC.1400.104. Moreover, all methods in the current study were performed according to the WHO (World Health Organization) guidelines and national regulations.

Not applicable.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Authors’ Contributions

ASD performed repellent assays. AA performed antibacterial tests. FK prepared nanogels. MS interpreted the ATR-FTIR spectra. GhGh contributed to the antibacterial assay and wrote the introduction. MO designed the study, analyzed the data, and drafted the MS. All authors contributed to the drafting of the manuscript and approved the final version.

Acknowledgments

This study was supported by Fasa University of Medical Sciences (400130).

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Copyright © 2022 Alireza Sanei-Dehkordi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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