COVID-19 Whole-Genome Resequencing with Redundant Tiling PCR and Subtract-Based Amplicon Normalization Successfully Characterized SARS-CoV-2 Variants in Clinical Specimens

Figure 5

Genome-wide amplicon bias in the 304 clinical samples. Distributions of counts for each amplicon from region_000 to region_147 among the 304 clinical samples are shown. Read numbers per 100,000 mapped reads were log10-transformed to calculate normalized count values. Amplicons with biotinylated primers are marked in blue (biotinylated). Amplicons without biotinylated primers are shown in red (normal). For each distribution, the box plot shows the 1^st quantile, median, and 3^rd quantile values.