International Journal of Spectroscopy / 2018 / Article / Fig 8

Research Article

Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities

Figure 8

(a) Agarose-gel electrophoresis diagram showing cleavage of pBR322 supercoiled DNA (200 ng) by complex 2 in presence of activators and reactive oxygen species at 310 K after incubation for 45 min. Lane 1: DNA control; Lane 2: DNA + C2 + Asc (0.4 M); Lane 3: DNA + C2 + H2O2 (0.4 M); Lane 4: DNA + C2 + MPA (0.4 M); Lane 5: DNA + C2 + DMSO (0.4 M); Lane 6: DNA + C2 + EtOH (0.4 M); Lane 7: DNA + C2 + NaN3 (0.4 M); Lane 8: DNA + C2 + SOD (15 units); Lane 9: DNA + C2 + methyl green (2.5 μL of 0.01 mg/ml solution); Lane 10: DNA + C2 + DAPI (8 μM). (b) A graph of percentage cleavage of DNA determined using image analyzer.
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