Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

Table 7

Predicted and actual results of the central composite design for CDW (g/L), PHA (g/L), and PHA (%).

Run order	A: Galactose (g/L)	B: NaCl (g/L)	CDW (g/L)		PHA concentration (g/L)		PHA content (%)
			Actual	Predicted	Actual	Predicted	Actual	Predicted
1	20	25		3.12		1.42		45.57
2	20	3.79		2.23		0.66		28.59
3	20	25		3.12		1.42		45.57
4	5.86	25		1.86		0.46		22.80
5	30	40		2.20		0.55		21.98
6	34.14	25		3.14		1.15		39.22
7	20	46.21		0.95		-0.04		0.27
8	30	10		2.92		1.05		35.06
9	10	40		1.30		0.06		3.43
10	20	25		3.12		1.42		45.57
11	10	10		2.02		0.56		30.40
12	20	25		3.12		1.42		45.57
13	20	25		3.12		1.42		45.57