Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T

Figure 3

(a) MFI of magnetic microspheres–-cTnT incubated with different concentrations of anti-cTnT at 25, 50, 100, 200, and 400 ng/mL, respectively. (b) MFI of coded conjugations incubated with different concentrations of IgG–FITC, i.e., 0.8, 1.0, 1.33, 2.0, 4.0, and 100 μg/mL or at dilution ratios of 1 : 2500, 1 : 2000, 1 : 1500, 1 : 1000, 1 : 500, and 1 : 20, respectively. (c) MFI of magnetic microspheres–cTnT incubated with anti-cTnT for 30, 60, 90, 120, and 150 minutes. (d) MFI of coded conjugate incubated with different IgG–FITC concentrations for 10, 30, 60, 90, and 120 minutes. (e) Incubation temperatures of the reaction system at a range of 4, 15, 25, 37, 50, and 60°C. (f) Binding response of cTnT in the samples or standard with anti-cTnT at the first reaction step.

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