Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis

Table 1

Bacillus cereus tsu1 cell propagation and PHB production on RCS substrate^†.

Items	Batch 1	Batch 2	Average
Cell biomass (mg/L culture )	575.17 ± 56.02	540.89 ± 18.43	558.03 ± 28.58
PHB mixture extract (mg/L)	139.44 ± 14.18	112.78 ± 12.54	126.11 ± 9.88
Pure PHB content (mg/L)	74.66 ± 8.54	80.61 ± 10.32	77.64 ± 6.45
PHB content in cell biomass (%)	13%	14.9%	13.95%
PHB conversion efficiency of rape seed cake (g/kg)	2.99	3.22	3.10

Bacillus cereus tsu1 was batch cultured for 24 hours in one-liter bottle containing (25 g/L) aqueous extracts of RCS. Two independent experiments each containing six replicates were conducted; PHB was extracted from dried bacterial cells. Six replicates were conducted; PHB content in crude extracts was determined by spectrophotometry analysis; PHB content measured using the spectrometric method is similar to the HPLC method (37).