Research Article
Effect of Cultivation Time and Medium Condition in Production of Bacterial Cellulose Nanofiber for Urease Immobilization
Table 3
Effect of different temperatures on immobilized urease activity.
| Enzyme | Immobilization matrix | Method of immobilization | Optimum temperature | Enzyme activity in optimum °C | Reference | 10°C | 20°C | 30°C |
| Urease | BCN (Medium B) | Cross-linking | 50°C | 98% | 91% | 79% | Present work | Urease | BCN (Medium A) | Cross-linking | 50°C | 84% | 72% | 61% | Present work | Urease | BCN (Medium C) | Cross-linking | 50°C | 88% | 65% | 53% | Present work | Urease | BCN (Medium D) | Cross-linking | 50°C | 94% | 81% | 73% | Present work | Urease | Electrospun polyacrylonitrile | Covalent bonding | 50°C | 60% | 45% | 30% | [28] | Urease | Nanostructured polymer | Covalent bonding | 30°C | 90% | — | — | [27] | Urease | Sodium alginate | Cross-linking | 50°C | 60 | 30 | — | [26] | Urease | Cellulosic cotton fibers | Covalent bonding | 35°C | 75 | — | — | [29] |
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