Peptide Inhibitor of Complement C1 Inhibits the Peroxidase Activity of Hemoglobin and Myoglobin

Figure 2

Comparison of PIC1 and ABAH inhibition of peroxidase activity of metHb, Hb from RBC lysates, and myoglobin. (a) 0.5 mg/ml metHb, (b) RBC lysates (1.25 × 10⁸ cells/ml equivalents), or (c) 0.5 mg/ml myoglobin were incubated with increasing concentrations of PIC1 and ABAH. Samples were then combined with TMB and absorbance was read at 450 nm. Data are means of three independent experiments ± SEM. (d) 0.5 mg/ml metHb was incubated with increasing concentrations of PIC1 and PEG24. Samples were then combined with TMB and absorbance was read at 450 nm.

(a)

(b)

(c)

(d)