A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites

<div>Preparation of hydroxyl-protected paromomycin. Reagents and conditions: (i) Boc<sub>2</sub>O (15 equiv), Et<sub>3</sub>N (12 equiv), MeOH, H<sub>2</sub>O, 24 h, 60°C, 64%; (ii) Ac<sub>2</sub>O (16 equiv), anh. pyridine, DMAP, 20 h, R.T., 78%; (iii) TFA/CH<sub>2</sub>Cl<sub>2</sub> (40%), 2 h, R.T., 100%.</div>

International Journal of Peptides

sch2

Scheme 2

Scheme 2: A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites 

Scheme 2 | A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites 