Research Article
Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila
Table 4
ELISA indicates that a netrin-like protein is secreted by Tetrahymena thermophila. A polyclonal anti-netrin-1 antibody showed reactivity in ELISA with whole cell extract as well as secreted protein obtained from Tetrahymena thermophila. Using a standard curve of netrin-1 concentration, we found that both total protein and secreted protein had a netrin-like peptide concentration of approximately 0.1 μM.
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