Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila

Table 4

ELISA indicates that a netrin-like protein is secreted by Tetrahymena thermophila. A polyclonal anti-netrin-1 antibody showed reactivity in ELISA with whole cell extract as well as secreted protein obtained from Tetrahymena thermophila. Using a standard curve of netrin-1 concentration, we found that both total protein and secreted protein had a netrin-like peptide concentration of approximately 0.1 μM.

Protein extract	Total protein concentration	Concentration of netrin-like peptide
Whole cell extract	114 mg/mL	0.1 μM
Secreted protein	0.29 mg/mL	0.1 μM