Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila

Figure 4

Tyrosine phosphorylation levels are not affected by netrin-1 peptide. Indirect immunofluorescence using PT-66 anti-phosphotyrosine antibody shows no difference in fluorescence intensity between control (a) and netrin-1 exposed cells (b). This indicates that tyrosine phosphorylation is not required for netrin-1 signaling. In contrast, cells stained with an anti-tubulin antibody (c) show a high level of fluorescence intensity.

(a)

(b)

(c)