TY - JOUR
A2 - Vaudry, Hubert
AU - Kuruvilla, Heather
AU - Schmidt, Bradley
AU - Song, Stephanie
AU - Bhajjan, Marian
AU - Merical, Matthew
AU - Alley, Caleb
AU - Griffin, Christopher
AU - Yoder, David
AU - Hein, Josephine
AU - Kohl, Daniel
AU - Puffenberger, Cambria
AU - Petroff, David
AU - Newcomer, Elise
AU - Good, Kortney
AU - Heston, Graham
AU - Hurtubise, Anna
PY - 2016
DA - 2016/03/31
TI - Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila
SP - 7142868
VL - 2016
AB - Netrin-1 is a highly conserved, pleiotropic signaling molecule that can serve as a neuronal chemorepellent during vertebrate development. In vertebrates, chemorepellent signaling is mediated through the tyrosine kinase, src-1, and the tyrosine phosphatase, shp-2. Tetrahymena thermophila has been used as a model system for chemorepellent signaling because its avoidance response is easily characterized under a light microscope. Our experiments showed that netrin-1 peptide is a chemorepellent in T. thermophila at micromolar concentrations. T. thermophila adapts to netrin-1 over a time course of about 10 minutes. Netrin-adapted cells still avoid GTP, PACAP-38, and nociceptin, suggesting that netrin does not use the same signaling machinery as any of these other repellents. Avoidance of netrin-1 peptide was effectively eliminated by the addition of the tyrosine kinase inhibitor, genistein, to the assay buffer; however, immunostaining using an anti-phosphotyrosine antibody showed similar fluorescence levels in control and netrin-1 exposed cells, suggesting that tyrosine phosphorylation is not required for signaling to occur. In addition, ELISA indicates that a netrin-like peptide is present in both whole cell extract and secreted protein obtained from Tetrahymena thermophila. Further study will be required in order to fully elucidate the signaling mechanism of netrin-1 peptide in this organism.
SN - 1687-9767
UR - https://doi.org/10.1155/2016/7142868
DO - 10.1155/2016/7142868
JF - International Journal of Peptides
PB - Hindawi Publishing Corporation
KW -
ER -