Modeling the QSAR of ACE-Inhibitory Peptides with ANN and Its Applied Illustration

Table 3

Reagents used in determination of ACE inhibiting activity.

	Blank (μL)	Sample (μL)
ACE (0.1 U/mL)	10	10
FAPGG (1 mmol/L)^1	50	50
HEPES buffer^2	40	0
Sample	0	40

¹FAPGG (1.0 mmol/L): prepared with 0.08 M HEPES buffer (pH 8.3) containing 0.3 M NaCl.
²HEPES buffer: HEPES 1.910 g, NaCl 1.755 g, dissolved with double-distilled water, pH adjusted with NaOH, and metered volume with double-distilled water to 100 mL, stored at 4°C.