Isolation and Characterization of a Defensin-Like Peptide (Coprisin) from the Dung Beetle, Copris tripartitus
Figure 2
Full-length cDNA sequence and comparison of amino acid sequences of insect defensins. (a) Nucleotide and deduced amino acid sequences of Coprisin gene isolated from C. tripartitus. The predicted amino acid sequence (single-letter abbreviation) is shown below the nucleotide within the open reading frame (ORF). The potential recognition sequence for the cleavage site within the constitutive secretory pathway (Arg-Xaa-Lys/Arg-Arg) is single-underlined. The putative mature peptide is boxed. Asterisk indicates the termination codon. Codons for initiation, termination, polyadenylation, and poly(A) tail are in bold. (b) Comparison of seven insect defensins sequences. Bars indicate gaps to optimize the sequence alignment. The six observed Cys(C) residues, which make up the disulphide bridges, are shaded grey. The GenBank accession numbers for the analyzed sequence are Anomala cuprea (BAD77966), Oryctes rhinoceros (BAA36401), Allomyrina dichotoma (AAB36306), Copris tripartitus (ABP97087), Thermobia domestica (CAM36306), Acalolepta luxuriosa (AAK35160), Tenebrio molitor (BAA04552), and Tribolium castaneum (XP968237).
