A Novel Innate Immune-Enhancement Strategy Combined with IVIG Rescues Mice from Fatal Staphylococcus aureus Septicemia
Figure 2
P4-mediated enhancement of OPK of S. aureus strainN with fresh PMNs isolated from human blood. In vitro opsonophagocytosis killing assay was performed with human peripheral blood PMN’s. Addition of P4 increased the opsonophagocytic killing of S. aureus strainN in the presence of strain-specific antibodies. Maximum enhancement (≥100%) was seen with HM904 over control that had all OPKA components except P4 (). Antibodies used in this in vitro assay: Gamunex (IVIG), a pooled human serum having reactivity with a wide range of pathogens including S. aureus; ab35194, rabbit polyclonal directed towards the soluble and structural antigens of the S. aureus; PAbClfA, rabbit polyclonal IgG directed towards the clumping factor A, ClfA of S. aureus, and HM904, humanized mouse monoclonal anti-ClfA IgG.
