A Novel Innate Immune-Enhancement Strategy Combined with IVIG Rescues Mice from Fatal Staphylococcus aureus Septicemia
Figure 1
P4-mediated enhancement of in vitro opsonophagocytic killing of Staphylococcus aureus strainN. In vitro opsonophagocytosis killing assay was performed with HL60-cells-derived granulocytes. Addition of P4 increased the opsonophagocytic killing of S. aureus strainN in the presence of strain-specific antibodies. Maximum enhancement (≥70%) was seen with HM904 over control that had all OPKA components except P4 (). Antibodies used in this in vitro assay: Gamunex (IVIG), a pooled human serum having reactivity with a wide range of pathogens including S. aureus; ab35194, rabbit polyclonal directed towards the soluble and structural antigens of the S. aureus; PAbClfA, rabbit polyclonal IgG directed towards the clumping factor A, ClfA of S. aureus, and HM904, humanized mouse monoclonal anti-ClfA IgG.
