Research Article
Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor
Table 2
Antimicrobial activities of DRS-DA2N and DRS-DA2NEQ on Gram-negative and Gram-positive strains.
| ā | Antimicrobial activity: MIC/MBC (pM) | DRS-DA2N | DRS-DA2NEQ | SAT | Melittin | MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC |
| Gram-negative strains | E. coli ATCC 8739 | 0.8 | 0.8 | 1.6 | 1.6 | >50 | >50 | 3.2 | 3.2 | E. coli K12 | 3.2 | 6.3 | 12.5 | 12.5 | n.d | n.d | 12.5 | 12.5 | E. coli ML35p | 0.8 | 0.8 | 1.6 | 1.6 | n.d | n.d | 12.5 | 12.5 | E. coli P7 (BLSE) | 1.6 | 12.5 | 12.5 | 50 | n.d | n.d | 6.3 | 6.3 | P. aeruginosa ATCC 9027 | 3.2 | 3.2 | 6.3 | 6.3 | >50 | >50 | 6.3 | 6.3 | P. aeruginosa ATCC 27853 | 12.5 | 50 | 50 | >100 | n.d | n.d | 50 | >50 | K. pneumoniae CIP 52.211 | 0.8 | 0.8 | 0.8 | 0.8 | n.d | n d | 12.5 | 12.5 | K. oxytoca CIP 7932 | 3.2 | 6.3 | 12.5 | >100 | n.d | n.d | zs | No | S. enterica CIP 8297 | 12.5 | 25 | 50 | 100 | n.d | n.d | 50 | >50 | Y. ruckeri ATGG 29473 | 3.2 | 12.5 | 25 | >100 | n.d | n.d | 6.3 | 12.5 |
| Gram-positive strains | S. aureus ATCC 6538 | 1.6 | 3.2 | 6.3 | 12.5 | >50 | >50 | 6.3 | 6.3 | S. aureus MRSA | 25 | >50 | 100 | >100 | n.d | n.d | 6.3 | 12.5 | S. aureus ST 1065 | 6.3 | 6.3 | 12.5 | 25 | n.d | n.d | 10 | 10 | S. epidermidis BM 3302 | 6.3 | 25 | 12.5 | >50 | n.d | n.d | 10 | 10 | L. monocytogenes SOR 100 | 25 | 25 | 100 | >100 | n.d | n.d | 1.5 | 3.2 | E. faecalis CIP A186 | 50 | >50 | 100 | >100 | n.d | n.d | 12.5 | 12.5 | L. garvieae ATCC 43921 | 25 | 50 | 100 | >100 | n.d | n.d | 6.3 | 25 | E. faecalis CIP 103015 | 6.3 | 6.3 | 12.5 | 12.5 | >50 | >50 | 1.6 | 1.6 | K. rhizophila ATCC 9341 | 0.8 | 1.6 | 1.6 | 1.s | n.d | n.d | 0.8 | 0.8 |
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