Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway

Figure 2

AP-Cav inhibits ERK1/2 phosphorylation. (a) After overnight low serum starvation, the sample was treated for 30 minutes with the AP-Cav peptide and then was stimulated with 50 ng VEGF/ml for 10 minutes. (b) After overnight low serum starvation, 30 minutes AP-Cav peptide treatment, and stimulation with 50 ng VEGF/ml for 2 hours, the ERK1/2 phosphorylation level was determined. Cells were treated with peptide for 30 minutes and then stimulated with 50 ng/ml of VEGF at the indicated times. At the end of the stimulation, cells were immediately lysed in RIPA buffer. The lysates were subjected to SDS-PAGE and western blot analysis with specific antibodies for phospho-ERK 1/2 and total ERK 1/2. Bar graph represents the phospho-ERK 1/2 to total ERK 1/2 ratio normalized to beta-actin levels. The data are mean ± s.e.m. from three independent experiments, .

(a)

(b)