-Equol, a Major Isoflavone from Soybean, Inhibits Nitric Oxide Production in Lipopolysaccharide-Stimulated Rat Astrocytes Partially via the GPR30-Mediated Pathway

Figure 3

Effect of S-equol on LPS-induced NO production, cell viability, and expression of iNOS protein. Cultured astrocytes were stimulated with 1 μg/ml LPS for 24 h in the presence of various concentrations of S-equol. The nitrite concentration in the medium was measured fluorometrically (a), and cell viability was evaluated with the MTT assay (b). The cells were treated with 1 μg/ml LPS in the absence or presence of 50 μM S-equol for 24 h. The expression of iNOS protein was detected with western blotting (c). Effect of isoflavones (S-equol, genistein, and daidzein, each concentration of 50 μM) on LPS-induced NO production (d). Data are the mean ± SEM of 5-6 samples. significantly different from 1 μg/ml LPS.

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