Limited Applicability of GW9662 to Elucidate PPARγ-Mediated Fatty Acid Effects in Primary Human T-Helper Cells
Figure 1
GW9662 exerts no effect up to 2 μmol/L and an additive effect on IL-2 inhibition in T-helper cells at 10 μmol/L. PBMC were pretreated for 30 min with GW9662 before 100 μmol/L c9,t11-CLA was added. After 19 h, cells were activated for subsequent 5 h. Intracellular IL-2 was flow cytometrically analyzed in lymphocytes gated for CD3 and CD4. . Data are expressed as means ± SEM of (a) and (b).
