Long-Lasting Effect of Infant Rats Endotoxemia on Heat Shock Protein 60 in the Pancreatic Acinar Cells: Involvement of Toll-Like Receptor 4
Figure 1
Western blot analysis of TLR4 protein level in the adult rat pancreatic acinar cells under basal conditions (lane 1), treated in the infant animals by lipopolysaccharide (Escherichia coli) at the doses of 5 mg/kg/day × 5 days (lane 2), 10 mg/kg/day × 5 days (lane 3), and 15 mg/kg/day × 5 days (lane 4) after 5 hours of incubation. Asterisk indicates significant () change, as compared to the control group. The blots were stripped and probed with GAPDH to document equal protein loading. All presented results were obtained in 4 consecutive experiments and are representative for the observed phenomenon.
