Inhibition of Gluconeogenesis by Boldine in the Perfused Liver: Therapeutical Implication for Glycemic Control

Figure 7

Concentration dependences of the effects of boldine on regulatory enzymes of the gluconeogenic pathway (a), ROS production (b), and mitochondrial respiration (c). Experimental procedures were described in the Materials and methods. (a) Values are of 4 to 6 assays. Asterisks () indicate statistical significance in comparison with the control condition (Student-Newman-Keuls, ). (b, c) Mitochondria of the rat livers were isolated as described in the Materials and methods. Values are of 4 to 6 assays. In (b), 0-rot: without boldine and rotenone; 0+rot: without boldine in the presence of rotenone; all boldine concentrations were evaluated in the presence of rotenone. The symbol # indicates statistical significance in comparison with 0-rot; indicates statistical significance in comparison with the stimulated condition (0+rot) (Student-Newman-Keuls, ).

(a)

(b)

(c)