Arsenic-Induced Injury of Mouse Hepatocytes through Lysosome and Mitochondria: An In Vitro Study

Figure 3

Alteration of arsenic-induced LMP evaluated by increase in green fluorescence as observed by confocal microscope using AO relocation technique in presence or absence of LMP inducer (BafA) and inhibitor (ChQ) at different time intervals. Control (a) and arsenic (b) exposed primary cultured mouse hepatocytes were preincubated with or without BafA (c) and ChQ (d) (magnification of all the figures are 40x). The figure is representative photomicrographs of 3 independent experiments. LMP was not observed in control hepatocytes since no significant green fluorescence was noted from 20 min to 75 min (ia-va). Arsenic exposure of mouse hepatocytes for 30 min only exhibited the appearance of green fluorescence which eventually increased with time (ib-vb). Pretreatment with BafA prior to arsenic treatment significantly prevented LMP as evidenced by negligible green fluorescence (ic-vc). LMP was further exacerbated following pretreatment with ChQ before arsenic treatment as depicted from 30 min of arsenic exposure in a time-dependent manner (id-vd).