Arsenic-Induced Injury of Mouse Hepatocytes through Lysosome and Mitochondria: An In Vitro Study

Figure 1

Arsenic-induced ROS formation in primary cultured mouse hepatocytes using DCF-DA. The results are expressed as independent experiments of 6 independent experiments. (a) Time-dependent arsenic (10 μM)-induced ROS in the hepatocytes was quantified as relative fluorescence intensity using multiplate reader. vs. control and vs. control. (b) The histogram represented ROS level produced by mouse hepatocytes in flow cytometry analysis with or without arsenic (10 μM) treatment at different time points. (c) Microscopically, intracellular ROS was evaluated at different time periods after staining with DCF-DA using confocal microscope. The representative figure showed that at 30 min of arsenic treatment, ROS generation was started from the peripheral region of a mouse hepatocyte and gradually progressed to the center of the cell with progression of time (40x magnifications). The figure is representative photomicrograph of 3 independent experiments.

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