Supplementation with Branched-Chain Amino Acids Induces Unexpected Deleterious Effects on Astrocyte Survival and Intracellular Metabolism with or without Hyperammonemia: A Preliminary In Vitro Study

Figure 1

BCAA induce cytotoxicity and reduce astrocyte viability. LDH cytotoxicity assay (a) and evaluation of viable cells (b) among astrocytes following treatment with 0-1 mM BCAA. HT22 cells (c) and HepG2 cells (d) were treated with 0.2 mM BCAA for 24, 48, and 72 h prior to calculating cell viability. LDH cytotoxicity assay (e) and evaluation of viable cells (f) among astrocytes, following treatment with 0.2 mM BCAA, 10 mM NH₄Cl, and BCAA+NH₄Cl cotreatments. Control: no treatment with either BCAA or other reagents. Data are presented as the ; ; .

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