Upregulation of miR-155 and -132 in Kupffer cells of alcohol-fed mice. Kupffer cells were isolated either from pair-fed or alcohol-fed mice (–14/group, cells from two mice were pooled) after 4 weeks of feeding. Cells were plated and after 2 h of incubation nonadherent cells were removed. Fresh medium was added to the adherent, and they were rested overnight. Next day, cells were washed with PBS and lysed with QIAzole (Qiagen). Total RNA isolated using the miRNeasy kit was used quantify miRNA as described above. Data represent mean values ± S.E.M. Statistical significance was determined using T-test (two-tailed).
