Induction of miR-155 in hepatocytes of alcohol-fed mice. Hepatocytes were isolated from the mice -6) after 4 weeks of feeding. Briefly, the livers were perfused, followed by digestion. Parenchymal cells (hepatocytes) were collected after low-speed centrifugation, washed, and were plated onto 6-well collagen-coated plates (BD-Biosciences). After 3 h, floating cells were removed, and adherent cells were washed twice with PBS and lysed in QIAzole (Qiagen). Total RNA was isolated and miRNA expression was quantified by TaqMan miRNA assays (Applied Biosystems). The data were normalized to SnoRNA202 (endogenous control) and shown as the fold change over the pair-fed control group. Data represent mean values ± S.E.M. Statistical significance was determined using non-parametric Mann-Whitney test.
