Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data

Figure 5

Phylogenetic tree of TLRs based on full-length amino acid sequences. Phylogenetic trees were constructed based on the amino acid sequences of TLRs from fish, birds, and mammals using the neighbor-joining method in MEGA 7. Numbers on the branches represent the percentage of bootstrap values. Node values represent the bootstrap confidence from 10,000 replicates. The GenBank accession numbers of the sequences are listed in Table 1. All TLRs were clustered into 4 groups, in which TLR15 and TLR4 were grouped separately (TLR15 group and TLR4 group), TLR1, 2, 6, 8, and 10 were clustered together (TLR1 group), and the remaining TLRs formed a large group (TLR3, TLR5M/S, TLR7, TLR8, TLR9, TLR11, TLR12, TLR13, TLR19, TLR20, TLR21, and TLR22). The large groups of 13 TLRs could be subdivided into 3 groups: TLR7, 8, and 9 clustered into one group (TLR7 subgroup); TLR3 and TLR5 clustered together (TLR3 subgroup); TLR11, 13, 19, 20, 21, and 22 are clustered into a group (TLR11 subgroup). Vertebrate TLRs were divided into six major TLR families (TLR1, TLR3, TLR4, TLR5, TLR7, and TLR11).