Enriching Genomic Resources and Marker Development from Transcript Sequences of Jatropha curcas for Microgravity Studies

Table 5

Summary statistics of SNP detection and validation.

	Depth		Number
SNPs discovered in the database	0		38,693
	≥4		7,584
	≥10		4,767
Chosen SNPs for validation	4–10		96
	11–20		90
	21+		89
Primers designed flanking the chosen SNPs		21
Total expected SNPs chosen		275
Amplified primers for Sanger sequencing		19
SNPs for Sanger sequencing		240
Primers succeeded for sequencing		17
SNPs succeeded for sequencing		214
Primers validated containing SNPs		5 (29.4%)
Matched SNPs with sequencing results		28 (13.1%)