International Journal of Genomics / 2017 / Article / Tab 5

Research Article

Enriching Genomic Resources and Marker Development from Transcript Sequences of Jatropha curcas for Microgravity Studies

Table 5

Summary statistics of SNP detection and validation.

DepthNumber

SNPs discovered in the database038,693
≥47,584
≥104,767
Chosen SNPs for validation4–1096
11–2090
21+89
Primers designed flanking the chosen SNPs21
Total expected SNPs chosen275
Amplified primers for Sanger sequencing19
SNPs for Sanger sequencing240
Primers succeeded for sequencing17
SNPs succeeded for sequencing214
Primers validated containing SNPs5 (29.4%)
Matched SNPs with sequencing results28 (13.1%)

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