Genetic Diversity and Population Structure of a Threatened African Tree Species, Milicia excelsa, Using Nuclear Microsatellites DNA Markers

Table 2

Estimates of single locus heterozygosity and genetic differentiation of the twelve Milicia excelsa populations over seven microsatellite loci.

Locus	A	AR	Pa (fq)	HO	HS	HT	𝐺ST	𝐹IS (‡‡)	𝐹IT (‡‡)	𝐹ST (‡‡)	𝑅ST (‡‡)
Mex163	26	7.50	5 (0.2)	0.765	0.873	0.929	0.061	0.121 (±0.06)***	0.180 (±0.06)***	0.067 (±0.01)***	0.082***
Mex63	32	7.69	8 (0.6)	0.506	0.835	0.941	0.113	0.357 (±0.07)***	0.440 (±0.06)***	0.129 (±0.02)***	0.692***
Mex51	16	6.65	1 (0.1)	0.554	0.770	0.900	0.144	0.277 (±0.04)***	0.388 (±0.03)***	0.154 (±0.02***	0.367***
Mex137	15	6.27	3 (0.1)	0.659	0.788	0.886	0.110	0.139 (±0.07)***	0.231 (±0.07)***	0.106 (±0.02)***	0.413***
Mex202	13	5.86	4 (0.2)	0.645	0.743	0.866	0.143	0.144 (±0.08)***	0.286 (±0.09)***	0.165 (±0.06)***	0.208***
Mex81	25	6.93	5 (0.4)	0.542	0.881	0.906	0.028	0.371 (±0.06)***	0.391 (±0.06)***	0.031 (±0.01)***	0.077**
Mex95	20	6.74	3 (0.2)	0.204	0.784	0.906	0.135	0.739 (±0.05)***	0.774 (±0.05)***	0.134 (±0.04)***	0.468***
Mean	147	6.81	29	0.554	0.811	0.905	0.104	0.308	0.385	0.112	0.342
(±SE)				(±0.07)	(±0.02)	(±0.01)	(±0.02)	(±0.08)***	(±0.07)***	(±0.02)***	(¶¶¶)
Overall ±SE
95% (§)								0.178 (±0.47)***	0.269 (±0.53)***	0.077 (±0.14)***	0.333 (¶¶)
99% (§§)								0.154 (±0.52)***	0.239 (±0.58)***	0.060 (±0.15)***	0.329 (¶)

Total number of alleles per locus (A); mean number of alleles per locus (AR); total number (Pa) and frequency (fq) of private alleles; observed heterozygosity (H_O); total gene diversity pooled overall population (H_T); mean genetic diversity within populations (H_S); proportion of the total genetic diversity that occurs among populations (𝐺_ST). Weir and Cockerham [19] estimates of F (𝐹_IT), Theta (𝐹_ST), f (𝐹_IS) (± SE) and 𝑅_ST; overall obtained after jackknifing over populations (‡‡), 𝑅_ST over loci following Rousset [22] (¶¶), following Goodman [23] (¶), and unweighted (¶¶¶); value obtained after bootstrapping over loci, number of replicates = 1000, with nominal confidence interval = 95% (§) and 99% (§§). Statistically significant levels of the tests are indicated by *(𝑃<.05), **(𝑃<.01) and ***(𝑃<.001).