Review Article
Label-Free Biosensors for Cell Biology
Table 1
Receptors, cell lines, and technologies and key points of the studies related to the use of label-free cellular assays for GPCRs.
| Receptors | Cells | Biosensors | Key findings | Ref |
| PAR2 Bradykinin B2 | A431 | DMR | Biosensor signal is originated from DMR | [8] |
| Adenosine A2B |
A431 |
DMR | Similarity analysis segregates ligands into clusters |
[38] | Bradykinin B2 | β2-adrenergic | EP4 | DMR signatures of distinct classes of GPCRs |
[92] | H1 | LPA receptors | P2Y1 | Integrative roles of adenylyl cyclases in GPCRs |
[93] | PAR1 | PAR2 | S1P receptors | VPAC1 | |
| P2Y1/2/11 | HEK293 | DMR | ECM coatings impact receptor signaling | [59] |
| LPA receptors | Porcine brain endothelial cells | ECIS | LPA increases tight junction permeability | [62] |
| PAR1 | Primary endothelial cells | ECIS | Thrombin promotes the formation of intercellular gaps | [64] |
| PAR1 | HMEC-1 | ECIS | Functional selectivity of PAR1 agonists | [65] |
| Bradykinin B2 | A431 | DMR | Systems cell biology of B2 receptor | [86] |
| PAR1 | A431 | DMR | HTS compatibility test | [87] |
| Endogenous receptors | HeLa U-937 U2OS TE671 | CDS | Receptor panning | [88] [89] |
| LPA receptors S1P receptors | Rabit corneal epithelial cell Rabit corneal endothelial cells | ECIS | The role of Gi signaling in cell monolayer permeability | [90] |
| Histamine H1 | CHO-H1 | RT-CES | Impedance signals were correlated with morphological changes |
[91] | Vasopressin V1a | 1321-N1-V1a | 5-HT1A | CHO-5HT1A | D1 | CHO-D1 |
| PAR1 PAR2 | A431 | DMR | Receptor cross-desensitization | [94] |
| Dopamine D2S | CHO-D2S | CDS | Ligand pharmacology characterization |
[95] | Muscarinic M4 | CHO-M4 | Dopamine D5 | CHO-D5 | CDS | Ligand-directed functional selectivity GPCR pleiotropic signaling |
[96] | Muscarinic M1 | CHO-M1 | Melanocortin MC4 | CHO-MC4 | HEK-MC4 | Cannabinoid CB1 | CHO-CB1 | Cannabinoid CB2 | CHO-CB2 |
| Histamine H1 β2-AR | A431 | DMR | Duplexed receptor assays for HT screening | [97] |
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