The DMR signal arising from epidermal growth factor (EGF) activated EGFR in native A431 cells is a systems cell biology readout of the EGFR. (a) Schematic drawing of EGFR signaling. (b, c, d) The sensitivity of the EGF DMR signal to different modulators, the two EGFR tyrosine kinase inhibitors AG1478 and BML-265 (b), the MEK1/2 inhibitor U0126 (c) and the PKC kinase inhibitor rottlerin (d). The control is the EGF response of cells pretreated with the assay vehicle only. In all experiments, EGF was at 32 nM, whereas the rest compounds used to pretreat the cells were at 10 μM.
