LncRNA X Inactive Specific Transcript Exerts a Protective Effect on High Glucose-Induced Podocytes by Promoting the Podocyte Autophagy via miR-30d-5p/BECN-1 Axis

Figure 5

XIST targeted miR-30d-5p to regulate autophagy-related proteins and autophagosomes in HG-induced podocytes. The experiments were divided into six groups. The first two groups of MPC5 cells were treated with NG or HG. The last four groups of MPC5 cells cotransfected with XIST overexpression plasmid/empty vector and miR-30d-5p mimic/mimic control were treated with HG. (a–d) The expressions of autophagy-related proteins (Beclin-1, LC3I/II, and ) in MPC5 cells were determined by Western blot. GAPDH was used as the internal control. (e) The autophagosomes in MPC5 cells were observed by the transmission electron microscope (magnification: 6,000x times; scale bar = 1 μm). The quantified values were presented as mean ± standard deviation of at least three independent experiments. ^∧, ^{∧ ∧ ∧} vs. vector + MC. ^#, ^### vs. XIST + MC. ^&, ^&&& vs. vector + M. HG: high glucose. NG: normal glucose. M: mimic. MC: mimic control. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; XIST: X inactive specific transcript.

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