Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells

Figure 4

Sestrin2 knockdown promotes TM-induced HepG2 cells apoptosis. (a) shRNA-mediated silencing of Sestrin2 in HepG2 cells was confirmed by qPCR analysis (one-way ANOVA, Dunnett t post hoc test; F_{(4, 15)} = 6.34, ). (b) Western blot analysis further validating Sestrin2 knockdown in HepG2 cells as determined by protein bands presented at 56 kDa. (c) Representative Western blot results of proteins involved with cell apoptosis. (d) Quantitative analysis of proteins involved with cell apoptosis (unpaired two-tailedt-test; for Bax/Bcl2: t₍₄₎ = −4.61, ; for C-caspase3/caspase3: t₍₄₎ = −4.15, ). (e) Representative images of TUNEL positive HepG2 cells in indicated groups. Scale bar, 50 μm. , , .

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