Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells

Figure 1

TM treatment-induced Sestrin2 up-regulation and ER stress. (a) mRNA level of Sestrin2 treated with various concentrations of TM (one-way ANOVA, Dunnett t post hoc test; F_{(5, 18)} = 7.62, ). (b) Representative western-blot results show Sestrin2 expression and proteins involving ER stress, such as GRP78, CHOP, XBP1s, and XBP1u. (c–f) Western blot quantitative analysis of Sestrin2 and proteins involving ER stress (one-way ANOVA, Dunnett t post hoc test; for Sestrin2: F_{(5, 6)} = 27.27, ; for GRP78: F_{(5, 6)} = 8.97, ; for CHOP: F_{(5, 6)} = 9.12, ; for XBP1s/XBP1u: F_{(5, 6)} = 20.27, ). (g) Viability of HepG2 cells with different TM concentrations treatment (one-way ANOVA, Dunnett t post hoc test; F_{(5, 18)} = 291.97, ). , , .

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