The Phosphatase PHLPP2 Plays a Key Role in the Regulation of Pancreatic Beta-Cell Survival

Figure 6

PHLPP2/1 knock-down does not restore insulin synthesis and secretion in INS-1 HG. (a) Insulin mRNA levels were assessed by real-time RT-PCR in mock-infected INS-1 HG, INS-1 HG AV-LD, and INS-1 HG AV-HD: n = 4. (b) Glucose-stimulated insulin secretion was assessed by measuring with a specific Elisa kit insulin concentration in medium obtained mock-infected INS-1 HG, INS-1 HG AV-LD, and INS-1 HG AV-HD exposed to increasing glucose concentrations (5.6, 11.2, 15.6, and 22.4 mM) for 20 minutes; n = 4.

(a)

(b)