The Phosphatase PHLPP2 Plays a Key Role in the Regulation of Pancreatic Beta-Cell Survival

Figure 4

PHLPP2/1 knock-down results in a significant improvement of insulin-stimulated phosphorylation of Akt, FoxO1, and mTor. (a) Representative western blot images of Akt phosphorylation on the Serine 473 residue (upper panel) and of total Akt levels (middle panel) in mock-infected INS-1 HG, INS-1 HG AV-LD, INS-1 HG AV-HD-stimulated (+I), or not (b) with 10⁻⁷ M insulin. Graphs of the mean changes over insulin-stimulated mock-infected INS-1 HG values INS-1 NG values of the densitometric values of pAkt obtained in 3 independent experiments and normalized for total Akt levels; indicates significant () differences for AV-infected INS-1 HG vs mock-infected INS-1 HG. (b) Representative Western Blot images of FoxO1 phosphorylation (upper panel), total FoxO1 levels (upper-middle panel), mTor phosphorylation (lower-middle panel), total mTor levels (lower panel) in mock-infected INS-1 HG, INS-1 HG AV-LD, INS-1 HG AV-HD-stimulated (+I), or not (b) with 10⁻⁷ M insulin. Graphs of the mean changes over insulin-stimulated mock-infected INS-1 HG values and INS-1 NG values of the densitometric values of pFoxO1 or pmTor obtained in 3–5 independent experiments and normalized for total levels of the unphosphorylated protein; indicates significant () differences for AV-infected INS-1 HG vs mock-infected INS-1 HG.

(a)

(b)