Research Article
The Phosphatase PHLPP2 Plays a Key Role in the Regulation of Pancreatic Beta-Cell Survival
Figure 3
Infection with shRNA constructs against PHLPP2/1 results in a significant knock-down of the expression of both isoenzymes. (a) Representative Western Blot images of PHLPP2 levels in INS-1 HG infected with increasing concentrations of the adenoviral vectors encoding for shRNA against PHLPP1/2 : 1.125 × 105 PFU (AV1.125); 2.250 × 105 PFU (AV2.250); 4.5 × 105 PFU (AV-LD); and 9 × 105 PFU (AV-HD). Control mock-infected cells were infected with empty AV constructs. Graphs of the mean changes over mock-infected INS-1 HG values of the densitometric values of PHLPP2 expression obtained in 4 independent experiments and normalized for β-actin levels; indicates significant () differences for AV-infected INS-1 HG vs mock-infected INS-1 HG. (b) Representative Western Blot images of PHLPP1 levels in INS-1 HG infected with 4.5 × 105 PFU (AV-LD) or 9 × 105 PFU (AV-HD) of the of the adenoviral vectors encoding for shRNA against PHLPP1/2 as compared to control mock-infected INS-1 HG cells. Graphs of the mean changes over mock-infected INS-1 HG values of the densitometric values of PHLPP1 expression obtained in 4 independent experiments and normalized for β-actin levels; indicates significant () differences for AV-infected INS-1 HG vs mock-infected INS-1 HG.
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