The Phosphatase PHLPP2 Plays a Key Role in the Regulation of Pancreatic Beta-Cell Survival

Figure 2

PHLPP1 and PHLPP2 expression is increased and Akt phosphorylation is reduced in INS-1 HG. (a) PHLPP1 and PHLPP2 mRNA levels were assessed by real-time RT-PCR (A), n = 5, indicates significant () differences for INS-1 NG vs INS-1 HG; (b) representative Western Blot images of PHLPP1 (upper panel) and PHLPP2 (lower panel) levels in INS-1 NG and INS-1 HG. Graphs of the mean changes over INS-1 NG values of the densitometric values of PHLPP1/2 expression obtained in 4 independent experiments and normalized for β-actin levels; indicates significant () differences for INS-1 NG vs INS-1 HG. (c) Representative Western Blot images of Akt phosphorylation on the Serine 473 residue (upper panel) and of total Akt levels (middle panel) in INS-1 NG and INS-1 HG stimulated (+I) or not (b) with 10⁻⁷ M insulin. Graphs of the mean changes over insulin-stimulated INS-1 NG values of the densitometric values of pAkt obtained in 4 independent experiments and normalized for total Akt levels. indicates significant () differences for insulin-stimulated INS-1 HG vs insulin-stimulated INS-1 NG.

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