miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity

Figure 5

ATG7 was directly downregulated by miR-129-5p in vitro. (a) Predicted target genes were validated by RT-qPCR in SVF white adipocytes. (b) Predicted target genes were validated by RT-qPCR in beige adipocytes. (c) miR-129-5p regulatory element in the 3′UTR of mouse ATG7 was identified by Targetscan. MRE, miRNA regulatory element; MRE-wt, wild-type MRE; MRE-mut, mutated MRE. (d) The effect of miR-129-5p mimics on the reporter construct containing ATG7-3′UTR in MRE-wt or MRE-mut as determined in HEK 293T cells. Relative luciferase units (RLU) are shown. (e–g) ATG7 and LC3I/II were determined by western blot transfected control and miR-129-5p mimics in mature white, beige, and brown adipocytes from SVF. Error bars represent SEM of more than three independent experiments ( and ).

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