miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity

Figure 4

Overexpression of miR-129-5p impaired brown adipocyte differentiation. (a) Oil Red O staining of mature brown adipocytes. (b) Relative TG content of the mature brown adipocytes. (c) The time course of miR-129-5p expression during normal brown adipogenic differentiation and the expression of regulators involved in adipogenesis were analyzed by RT-qPCR. (d) Protein levels of the target genes were determined by western blot. (e) Densitometry quantification of western blot in (d). The SVF from interscapular fat tissues was induced to differentiate toward the brown adipocytes. Data were analyzed with Student’s t-test and were presented as mean ± SEM of a representative of more than three independent experiments (, , and ).

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