miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity
White adipocyte differentiation impaired by microRNA mimic-mediated overexpression of miR-129-5p. (a) Expression levels of miR-129-5p. (b) Oil Red O staining of mature adipocytes. The top two images were captured by a camera; the lower two images were acquired with a microscope at 100x amplification. (c) Relative TG content of cells isolated from EWAT. (d) The time course of miR-129-5p expression during normal adipogenic differentiation was detected, and related white adipogenic genes were qualified by RT-qPCR. (e) Protein levels of the target genes were determined by western blot. (f) Densitometry quantification of western blot. The results of Student’s t-test are presented as mean ± SEM of a representative of more than three independent experiments (,, and ).